Certificate of Analysis

Quality Assurance Document

AvoBio

Molecular Biology Reagents

Product Information

Product Name

pUC19

Catalog Number

P001

Lot Number

LOT-P001-2026

Technical Specifications

Vector BackbonepUC19

Selection MarkerAmpicillin (100 µg/mL)

PromoterLac (inducible)

Copy NumberHigh

Host StrainDH5alpha, JM109, TOP10

GC Content50.7%

Quality Control Test Results

Test	Specification	Result	Status
DNA Purity (A260/A280)	1.8 - 2.0	1.85	Pass
Supercoiled Content	≥ 90%	>95%	Pass
Endotoxin Level	< 0.1 EU/μg	< 0.1 EU/μg	Pass
Sequence Verification	100% match	Verified	Pass
Restriction Digest	Expected pattern	Confirmed	Pass
Sterility	No growth	No growth detected	Pass

Storage & Handling

Storage Conditions

• Store at -20°C for long-term storage
• Stable at 4°C for up to 1 month
• Avoid repeated freeze-thaw cycles
• Protect from light

Handling Precautions

• Use sterile technique when handling
• Thaw on ice before use
• Centrifuge briefly before opening
• Use nuclease-free pipette tips

Certification

This product has been manufactured and tested in accordance with AvoBio's quality management system. All tests were performed using validated methods and calibrated equipment. This certificate confirms that the above lot meets all specified quality standards at the time of release.

Quality Assurance Manager

Date: January 9, 2026

Document ID: COA-P001-LOT-P001-2026

Disclaimer: This product is for Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The information provided in this certificate is accurate to the best of our knowledge at the time of release.

AvoBio Inc.

Seattle, WA | [email protected] | www.avobio.com